No Stain Analysis: Eliminate cell staining for cell counting and confluence measurements using. . 1 and above:. 蛍光 発光 ELISA. Molecular Devices’ ScanLater Western Blot Detection Cartridge uses TRF detection to turn its SpectraMax i3X and Paradigm plate readers into Western blot readers. Additionally, user-installable options allow the SpectraMax i3 System to. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. . My proficiency in techniques such as •PBMC isolation, counting and. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyMeasure oxidative metabolism and glycolytic activity on the SpectraMax i3x reader. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. Table 3 Optimal instrument settings for Valita Titer Plus assay Fluorescence Polarization measurement on Molecular Devices microplate readers. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection. Citation 2002). 과 같은 Parameter를 검사하여 평가할 수 있습니다. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. The TUNE detection cartridge uses a breakthrough monochromator design that enables researchers to easily optimize their. Read height should be optimized for . HTRF-compatible SpectraMax Multi-Mode Readers. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. Version 7. A SpectraMax ® i3x was used to determine uo-rescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. compound concentration, and an IC 50 value of 21. 2 Parallel Line Analysis Conc log [ng/ML] RLU. Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. (SpectraMax i3/i3x) CatchPoint SimpleStep ELISA (SpectraMax Paradigm) SpectraDrop. The StemoniX microBrain 3D platform is a homogenous 3D neural spheroid system in 96 and 384-well format. 価格、見積もり、お近くの販売店を. 100 fmol 1536-well (8 µL) 0. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. With optimized reagents, option to operate the readers. Quantification of NETs was performed with the SpectraMax i3x microplate-reader (Molecular Devices, United Kingdom). The Results. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. Molecular Devices Software. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. SpectraMax i3x; SpectraMax iD3 / iD5; SpectraMax M Series; SoftMax Pro Software; CELLULAR IMAGING. 0 mm hex key to remove the six blue retaining screws that secure the transport bracket (B) to the instrument. AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. Western blot assay 5 x 105 cells were seeded in 24-well plates in 500 μl growth medium and stimulated with novel compounds at 10μM. Background noise is reduced by the reader’s cooled PMT, enabling increased sensitivity and dynamic range. The NET-specificity of the detected signal was determined by incubation with DNase I (50 U/mL, Qiagen, Germany). SpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax. Data analysis. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. 302 low ref light. . The SpectraMax i3x is a multi-mode microplate reader that measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell imaging, and fast kinetics. 10. ZERO BIAS - scores, article reviews, protocol conditions and more. The protocols are also available on the SoftMax Pro Software protocol sharing. SpectraMax i3x microplate reader and SoftMax Pro Software protocol. . Price from $9. Use the 3. i3x single use: $35: $52. 2 Note Section Patch:1) Select the correct plate and the wells that you want to measure. . The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. 303 unable to cal dark current. Average 98 stars, based on 1 article reviews. . For more detailed information and technical support of Molecular Devices instruments or software,The SpectraMax i3x Multi-Mode microplatereader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging and Fast Kinetics with Injectors. Explore more about Optimized settings for Transcreener TR-FRET assay on SpectraMax iD5 and SpectraMax i3x readers. Spectranet customer portalSpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Seller Details. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Both Microplate Optimization and Read Height Adjustment were. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. The plate was shaken and incubated in the dark for 10 minutes. SpectraMax i3x Multi-Mode Detection Platform. Insert the snorkel into the bottle and then insert the snorkel into the snorkel clamp. Using the SoftMax Pro Workflow Editor, a kinetic cycle containing an absorbance read (PlateOD600) and a fluorescence read (PlateGFPBottom) with a 5-second linear plate shake between reads was created (Figure 1). Multimode Readers Comparison Chart. Learn more about its features, specifications, and applications in this user guide. Video: SpectraMax i3x Overview; Brochure: SpectraMax i3x Multi-Mode Detection PlatformVersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. As per Volza’s India Import data, Spectramax import shipments in India stood at 365, imported by 145 India Importers from 28 Suppliers. spectramax i3x - by Bioz Stars , 2023-11. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. ai; ImageXpress Pico; IN Carta Image Analysis Software; CLONE SCREENING. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. Introduction Inflammation, the body’s reaction to infection, irritation, or other injury, is accompanied by increased endothelial chemokine. 3. User-upgradeable detection modules allow you to expand the capabilities of your microp. Eight different automated tests are provided: • Absorbance accuracy (linearity) • Precision (reproducibility) • Stray light • Wavelength accuracy • Wavelength repeatability SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. SpectraMax iD3 and iD5 Register or Sign In. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Download PDF. Eliminate time-dependent substrate addition steps. Products : SpectraMax i3x reader, SoftMax Pro GxP Software, SpectraMax i3, SoftMax Pro Software, SpectraMax MiniMax cytometer When I attempt a read with my SpectraMax i3 or i3x reader using the injector cartridge, why do I see a plate height error, even though I have entered the correct plate height?. Structured Review. SpectraMax Paradigm Platform in conjunction with AlphaLISA provides a powerful tool to screen novel therapeutics in a pathologically relevant, endothelial cell-based model. GFP expression imaged on the cytometer. 7. The CellTiter-Glo assay from Promega uses luciferase. MC38 in vivo tumor model in hPD-1. Page 9 Setting Up the SpectraMax® MiniMax™ 300 Imaging Cytometer 2. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex. Open the catalog to page 1. Published Dec 16, 2020 | Updated Oct 10, 2023. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area sensor for temperature control. . 315 can't find zero order. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. 313 reference gain check fail. RIDASCREEN Gliadin competitive ELISA. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. com The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. ZERO BIAS - scores, article reviews, protocol conditions and more. The SpectraMax MiniMax 300 Imaging Cytometer option is a field-upgradeable option for the SpectraMax i3/i3x Multi-Mode Detection Platform that offers cellular imaging capability. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles). 2. The SpectraMax® i3x reader allows you to. Data were analyzed with SoftMax ® Pro Software and graphed using a 4-parameter curve fit. com Check our website for a current listing of worldwide distributors. Contact Us Phone: +1. . EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Description 1. 98 / 100 stars. Transfected cells were imaged on the SpectraMax MiniMax 300 Imaging Cytometer. Examples: “Press carefully to engage,” “ Do not. Database contains 1 Molecular Devices SpectraMax i3x Manuals (available for free online viewing or downloading in PDF): Installation manual . Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. User Manuals, Guides and Specifications for your Molecular Devices SpectraMax i3x Measuring Instruments. Table 1. Figure 3. . Version 7. StakMax Handling Stacker Base Unit. Bioz Stars score: 98/100, based on 1 PubMed citations. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. each new assay. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. The SpectraMax i3x reader offers a modular design allowing the addition of FP detection to the instrument in just minutes. Concentrating the excitation light on the sample through label specific optics, high levels of sensitivity and performance are reached. 0´ 104 1. Downloads. iD5 reader (the SpectraMax iD3 reader is also suitable for these assays, as it has the same onboard fluorescence performance as the SpectraMax iD5 reader), SpectraMax i3x, SpectraMax M4 or M5, and FilterMax F5 Multi-Mode Microplate Readers. SpectraMax® L reader support; ü. . Microplate optimization should be performed the first time the assay is run, and subsequently for . The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. Finally, their fluorescence was measured with 365-nm excitation and 450-nm emission filters using a microplate fluorometer (SpectraMax i3x, Molecular Devices, San Jose, CA, USA) (Saiya-Cork et al. SpectraMax Microplate Readers. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added. Background Parallax,Columns 2. Benefits • User-upgradeable application modules including cellular imaging • Sensitivity across spectrum with Spectral Fusion™ Illumination • Expanded dynamic range with cooled PMT • Control and analytics provided by SoftMax® Pro Software SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. 0. Setting iD5 i3x M5 Paradigm Measurement mode Fluorescence Polarization Fluorescence Polarization Fluorescence Polarization Fluorescence Polarization Excitation 485 nm monochromator 485 nm 485 nm 485 nmPage 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. One sensor measures the temperature of the top chamber heating foil, and another is used to measure the bottom chamber heating foil. 0´ 104 6. Bioz Stars score: 98/100, based on 1 PubMed citations. The SpectraMax DuoLuc Reporter Assay has been performance optimized on the SpectraMax i3x reader with Injector Cartridge. Antigen Quantitation Protocol. In this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. ATP LLD (pM) ATP LLD (moles) SpectraMax i3x, luminescence . The soil suspension (600 µL) and the substrate (L-3,4-dihydroxyphenylalanine, DOPA, 150 uL, 25 mmol L -1 ), were mixed and then added to. . Contact Local Team. Failed to load PDF file. 100 fmol 384-well (75 µL) 0. SoftMax Pro Software enables users to quickly set up analysis of cell counts via a simple point and click user interface. SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. Curves were forced through x,y = 0. BMG LABTECH VANTAstar. Expand your lab’s capabilities to include flash applications such as dual luciferase and ATP assays with the SpectraMax® i3x injector module with SmartInject® Technology ensuring equal mixing across the plate for high-precision experiments. When used in combination with the SpectraMax Injector Cartridge, this system enables a wealth of flash type luminescence applications including reporter gene and other assays with exceptional sensitivity and dynamic range. Enzyme Activation Analysis;. Plaque Reduction Neutralization Test (PRNT) SARS-CoV-2 neutralization was assessed as previously described . The SpectraMax i3x reader allows you to unravel the mysteries of science by exploringLuciferase activity was measured using a SpectraMax i3x microplate reader (Molecular Devices). The SpectraMax M4 reader had identical performance specifications to the SpectraMaxLuminescence was read on the SpectraMax i3x reader and the SpectraMax L reader. Figure 4. Spectranet customer portal. . Download SoftMax® Pro 7. Thetypeof luciferase(Renilla. SpectraMax® Microplate Readers and SoftMax® Pro Software. SpectraMax i3x; SpectraMax iD3 / iD5; SpectraMax M Series; SoftMax Pro Software; CELLULAR IMAGING. Spectranet customer portalThe SpectraMax i3x reader and MiniMax cytometer can be used with FluoroBlok cell culture inserts to directly count cells from high-quality images to measure cellular migration over time. SpectraMax i3x readers. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when. SpectraMax i3x Multi-Mode Microplate Reader. Serial number 33 900 01-3387. Instrument. Materials and methodsSpectraMax i3x Multi-Mode Microplate Reader. Monochromator optics with adjustable bandwidth offer excellent sensitivity down to 6 nM tryptophan, as well as precise determination of the fluorescence emission peak shift that occurs upon protein denaturation. In this product video, see how the SpectraMax M5 quickly reads samples. 吸収度マイクロプレートリーダー SpectraMax® Mini. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 660-665 Eskdale Road. Real-time measurement of extracellular oxygen depletion and acidification in cultured cells and microorganisms. Prime function 20 amol. D 1 1. ; The top 3 importers of Spectramax are India with 365. Published Dec 16, 2020 | Updated Dec 16, 2020. to View Figures. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). . Monday to Friday 8am - 5pm GMT. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. . . Faster data acquisition and analysis with preconfigured SoftMax Pro protocols. Ease of use Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. The SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. Spectranet customer portal Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. Description 1. 1. Subsequently, 100 μL of the substrate solution (2 mM of p-nitrophenyl butyrate in dimethylformamide) was added into the mixture, and incubated at 37°C for 15 min. 1-xiAt last, the relative absorption was estimated using a microplate reader (SpectraMax i3x, Molecular Devices, LLC, Sunnyvale, CA, USA) at 450 nm. GM3000 Register or Sign In. 303 unable to cal dark current. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. 99 to $1999. 306 plate air cal fail,low light. ü; Instrument Diagnostics Mode (for SpectraMax® i3x, SpectraMax® iD3, SpectraMax® iD5, SpectraMax® Paradigm)Absorbance was measured on the SpectraMax i3x Multi-Mode Platform at 475 nm and 660 nm after 1-4 hours of incubation. 4. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence,. 1. Find regional support. Calcium. . With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. After 2 days of incubation, LUC and REN activities were measured using a SpectraMax ® i3x Multi-Mode detection platform (Molecular Devices, USA) with a Dual-Luciferase Reporter Assay Kit (Promega. Spectramax I3x, supplied by Danaher Inc, used in various techniques. . Summary of Contents for Molecular Devices SpectraMax M2. viii SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual Conventions Used in this Manual The names of keys that appear on the SPECTRAmax 340PC control panel are shown in boxed Helvetica type. Supplier: Molecular Devices. . The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. 5mM EDTA, 1% NP40,Explore a wealth of applications with the future-ready SpectraMax i3x. モジュールを追加してアップグレードすることにより、ウェスタンブロットやイメージング、試薬インジェクタを用いた高速カイネティック測定を行う. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. 7 Figures from 1126 Publications. . Molecular Devices are known for both the reliability and the wide uses of their products. (3) Molecular Devices, SpectraMax i3x, Microplate Readers (2) 2022 (1) 2020 Invetech Korus KRS-100 Elutriation & Washing Systems (3) 2021 ThermoFisher Scientific, invitrogen, CytKick Max AutoSampler w/ Attune NxT: Acea NovoCyte Flow Cytometer: Appliedbiosystems, QuantStudio 7 Flex, Real-Time PCR SystemThe SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. I am a person who can handle a range of laboratory and data management tasks with precision and accuracy. Learn how you can perform flash-based applications, such as dual luciferase and ATP assays, with high precision and accuracy using this cartridge. 0´ 105 dsD NA (p g/ wel ) R F UFluorescence intensity was measured using a Spectramax i3x plate reader (Molecular Devices) with excitation at 575 nm (bandwidth 9 nm) and emission at 608 nm (bandwidth 15 nm). 304 signal level saturation. Description 1. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. . The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. All test measurements and calculations are handledPreconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. SpectraMax MiniMax 300 细胞成像系统 为 SpectraMax i3x 多功能微孔板检测平台而打造！ • • • • 主要特点 系统全面兼容细胞成像 及微孔板检测技术 独特无标记分析技术无需 对细胞染色即可进行细胞 计数和贴壁度的计算 具有多种检测功能可对 细胞活力及毒性进行全. The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube (PMT) for improved detection in extremely. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideRecommendations for SpectraMax i3/i3x plate readers The Molecular Devices SpectraMax i3/i3x allows use of the pH-Xtra Glycolysis Assay with time-resolved fluorescence (TRF) detection. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. With optimized reagents, option to operate the. Both readers demonstrated their ability to detect the HTRF signal from the terbium donor and red acceptor combination, and they could be used for saturation studies using the Tag-lite technology. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. SpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. , Fisher Scienti fi c, cat. 5 Figures from 202 Publications Thermo Fisher Scientific. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. SpectraMax i3x Multi-Mode Microplate Reader; SpectraMax MiniMax 300 Imaging Cytometer *Here, the NucView 488 with DMSO formulation was used. 312 gain calibration failed. Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. However, no piece of equipment is immune to breaking down. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree Technology Measure oxidative metabolism and glycolytic activity on the SpectraMax i3x reader. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. . For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. Molecular Devices. SpectraMax i3x, onboard luminescence 121. Keywords: htrf spectramax detection cartridge, spectramax i3, spectramax paradigm system, htrf reagents, spectramax i3 and paradigm readers, HTRF assay, htrf. Read more about HTRF SpectraMax Detection Cartridges for SpectraMax i3x & Paradigm Systems which utilize a high energy Xenon flash lamp for the sensitive reading of HTRF reagents. The SoftMax Pro Software provides integrated instrument control. 313 reference gain check fail. i3. 1321N1, a human astrocytoma cell line. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. 性能特点MD SpectraMax i3X 多功能荧光检测仪为采用光谱融合照明设计、测试功能模块化卡盒设计，具有细胞成像分析单元。. A: percent GFP-positive cells calculated using the classification feature in the software. SoftMax Pro. 01 0. 0´ 104 8. We would like to show you a description here but the site won’t allow us. 020 fmol 0. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,(foc) spectramax i3x alphascreen 384 std detection cartridge - p/no:0200-7017 (cartridge for demonstration purpose) united kingdom: chennai air cargo: unt: 1: 1,254,478: 1,254,478: aug 04 2016: 90273090: spectramax i3x multi-mode microplate reader; iqoq for spectramax i3x smp7 gxp license(for r&d lab use only) united states: bombay air cargo. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. We would like to show you a description here but the site won’t allow us. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. 7 mm for all shake intensities. The spectra analysis and K d were determined using Sigmaplot 14. The plate was shaken and incubated in the dark for 10 minutes. ü; ü. ü; ü. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. ZERO BIAS - scores, article reviews, protocol conditions and moreThe SpectraMax ® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and. 더 많은. GloMax Discover System. NORTH AMERICA. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Cet appareil ISM est confomre à la norme NMB-001 du Canada. Nucleic Acid and Protein Quantitation. SpectraMax i3x Register or Sign In. This technique allows for quality control and assurance when performing surface functionalization of nanoparticles. SpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Figure 2-1 Label on Rear of Instrument WARNING!We would like to show you a description here but the site won’t allow us. . Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. 304 signal level saturation. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. 99. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. 312 gain calibration failed. SpectraMax® ABS and ABS Plus reader support; ü. The SpectraMax i3x Multi-Mode Detection Platform User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax i3x, a versatile and customizable microplate reader that can perform various assays and imaging tasks. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any application or budget. The assay can accurately test the toxicology of compounds as shown in Figure 1. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Asterisks indicate the dilution of assay control that should be minimally detectable for each kit. SpectraMax i3x Multi-Mode Detection Platform User Guide. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. Bioz Stars score: 98/100, based on 1 PubMed citations. After 2 hours of treatment cells were harvested by centrifugation and cell pellets were lysed in RIPA buffer (20mM Tris-Cl, 150mM NaCl, 0. com. Fractional occupancy Corrected BRET ratio NanoBRET. . Italic and boldface type are used for emphasis. Binding and Inhibition. . Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. With a preconfigured protocol to simplify data acquisition and analysis, SoftMax Pro Software completes this total solution for fast, sensitive, and reliable dual luciferase reporter assay results. Find regional support. 1 software Download page. Microplate readers, multi-mode, SpectraMax® i3x. Microplate Readers SpectraMax. The Immunochemical Core includes approximately 2,600 square feet of space in the Joseph Building at Mayo Clinic Hospital, Saint Marys Campus. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Explore a wealth of applications with the future-ready SpectraMax i3x. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries.